15 Ituaiga ole Human Papillomavirus E6/E7 Gene mRNA
Igoa o oloa
HWTS-CC005A-15 Ituaiga ole Human Papillomavirus E6/E7 Gene mRNA Su'esu'eina Kit (Fluorescence PCR)
Epidemiology
O le kanesa o le faa'au'auna o se tasi lea o ituaiga e sili ona taatele o le kanesa o tama'ita'i i le lalolagi atoa, ma o lona tulai mai e vavalalata ma le papillomavirus o tagata (HPV), ae na'o sina vaega itiiti o fa'ama'i HPV e mafai ona tupu ma kanesa.O le HPV maualuga e ono a'afia ai sela epithelial cervical ma maua ai ni oncoproteins e lua, E6 ma le E7.O lenei polotini e mafai ona a'afia ai le tele o polotini feavea'i (e pei o le tumo suppressor proteins pRB ma le p53), faaumiumi le taamilosaga o le sela, aafia ai le DNA synthesis ma genome stability, ma fa'alavelave i le antiviral ma antitumor immune responses.
Auala
| Auala | Vaega | Su'e le genotype |
| FAM | HPV Reaction Puffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Gane β-actin tagata | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Gane INS tagata |
Vaega Fa'atekinisi
| Teuina | Suavai: ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Usu fa'a'au'au |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kopi/mL |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR SystemFa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Reagent fa'atonuina e aveese ai: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) e le Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona fa'atinoina le fa'atonuga mo le fa'aoga. .Ole fua ole elution fautuaina ole 50μL.Afai e le'i mae'a atoa le fa'ata'ita'iga, toe fa'afo'i i le Laasaga 4 mo le toe fa'amama.Ona faataitai lea e tusa ai ma faatonuga mo le faaaogaina.
Fa'atonuga fa'afo'iina: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).E tatau ona faʻatautaia le faʻaaogaina e tusa ai ma faʻatonuga mo le faʻaaogaina faʻamalosi (I le laasaga 5, faʻaluaina le faʻaogaina o le DNaseI fofo galue, o lona uiga, ave le 20μL o le RNase-Free DNaseI (1500U) faʻasoa faʻaputu i totonu o se fou RNase-Free centrifuge tube, faaopoopo le 60μL o le RDD buffer, ma faʻafefiloi malie).Ole fua ole elution fautuaina ole 60μL.Afai e le'i mae'a atoa le fa'ata'ita'iga, toe fa'afo'i ile Laasaga 5 mo le toe fa'amama.Ona faataitai lea e tusa ai ma faatonuga mo le faaaogaina.
