12 ituaiga o siama o le manava
Igoa o le oloa
HWTS-RT071A 12 ituaiga o Pusa e iloa ai siama o le manava (Fluorescence PCR)
Alavai
| Alavai | hu12Tali Fa'apa'u A | hu12Tali Fa'apa'u B | hu12Tali Fa'apa C | hu12Tali Fa'aputu D |
| FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
| VIC/HEX | Pulega i Totonu | Pulega i Totonu | HPIV II | Pulega i Totonu |
| CY5 | IFV A | MP | HPIV III | / |
| ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Fa'atulagaga Fa'apitoa
| Teuina | ≤-18℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'isu'i o le gutu (oropharyngeal swab) |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2：300 Kopi/mLsiama fulū B：500 Kopi/mLsiama fulū A：500 Kopi/mL Adenovirus：500 Kopi/mL mycoplasma pneumoniae：500 Kopi/mL siama o le respiratory syncytial：500 Kopi/mL, siama parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): 500 Kopi/mL Rhinovirus：500 Kopi/mL siama metapneumovirus o le tagata：500 Kopi/mL |
| Fa'apitoa | O le suʻesuʻega o le cross-reactivity ua faʻaalia ai e leai se cross-reactivity i le va o lenei kit ma le enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-herpes zoster virus, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans ma le human genomic nucleic acid. |
| Meafaigaluega Fa'aoga | Fa'aogaina o Biosystems 7500 Faiga PCR Taimi MoniFa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi MoniQuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Polokalame PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Faiga PCR Taimi Moni, Polokalama Iloa PCR Taimi Moni a le LineGene 9600 Plus（FQD-96A，Tekonolosi Hangzhou Bioer) MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni (Suzhou Molarray Co., Ltd.) BioRad CFX96 Polokalame PCR Taimi Moni BioRad CFX Opus 96 Polokalame PCR Taimi Moni |
Tafega o Galuega
Filifiliga 1.
O le vaila'au e fa'aaogā mo le fa'amamāina ua fautuaina: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) mai Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., E tatau ona faia le aveeseina o le ea e tusa ai ma faatonuga.
Filifiliga 2.
O le vaila'au e fautuaina mo le fa'a'esea: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) mai Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., e tatau ona faia le fa'a'esea e tusa ai ma fa'atonuga ma le fa'aeteete. O le aofa'i fautuaina o le elution e 80μL.
Filifiliga 3.
O le vaila'au e fa'aaogā e aveese ai le vai: Nucleic Acid Extraction po'o le Purification Kit (YDP315) mai le Tiangen Biotech (Beijing) Co., Ltd., e tatau ona faia le fa'aaogāina e tusa ai ma fa'atonuga ma le fa'aeteete. O le aofa'i fautuaina o le elution e 100μL.
